Normal Phase Chromatography

Normal-phase chromatography employs a polar stationary phase and a non-polar mobile phase to separate compounds based on their polarity. Less polar compounds are eluted first, followed by more polar compounds, enabling effective separation.

This technique is widely used in HPLC, versatile for separating water-sensitive substances, geometric isomers, cis-trans isomers, and chiral compounds. It is valuable for pharmaceutical research, natural product analysis, and environmental monitoring.